ABSTRACT: 0912

Extrinsic Tooth Stain Removal with Proteases

C.J. MORDAS¹, A. MEHTA¹, M. MOORE², and R.J. GAMBOGI¹, ¹Johnson & Johnson Healthcare Products, a Division of McNeil PPC, Skillman, NJ, USA, ²Indiana Purdue University Fort Wayne, USA

Objective: Evaluate both plant (papain, bromelain, ficin) and animal (trypsin) proteases for their potential to remove extrinsic staining on enamel. Methods: Bovine specimens were treated with a staining broth containing human saliva for 10 days. After complete staining and buildup of a protinaceous layer, the specimens were measured for L*a*b* using a SpectroShade Micro (MHT, Italy). The specimens were then treated for thirty minutes at 37°C with 5% active enzyme or control solutions at optimum pH(6-8). L*a*b* values were measured again after treatment was complete.

Results:

Change in Spectrophotometer Readings^†

Total Change in Stain

Sample

ΔL*

Δa*

Δb*

ΔE

Control (Buffer)

0.50 ± 0.31^A

0.01 ± 0.57^A

0.20 ± 0.25 ^A,B

0.84 ± 0.21^A

Papain

1.95 ± 0.94^B

-0.08 ± 0.41^A

0.43 ± 0.51^A

2.13 ± 0.84^B

Bromelain

2.14 ± 0.62^B

-0.24 ± 0.65^A

0.33 ± 0.72^A,B

2.37 ± 0.62^B

Ficin

2.18 ± 0.72^B

-0.55 ± 0.31^A

0.18 ± 0.65^A,B

2.36 ± 0.70^B

Trypsin

1.44 ± 0.44^B

-0.14 ± 0.62^A

-0.29 ± 0.66^B

1.68 ± 0.55^B

Polyphosphate Rinse

2.11 ± 0.99^B

-0.26 ± 0.68^A

-0.25 ± 0.78^B

2.36 ± 1.00^B

^†Mean ± standard deviation, n=14. For each variable, group values with a different letter were statistically different at p<0.05 using ANOVA.

Conclusions: All enzymes tested exhibited statistically significant increased stain removal compared to the negative control and comparable activity to the polyphosphate positive control. Papain, bromelain, and ficin exhibited directionally increased stain removal when compared to trypsin, although not statistically significant.

Seq #134 - Whitening/Stain/Bleaching/Dentifrices
2:45 PM-3:45 PM, Friday, April 4, 2008
Hilton Anatole Hotel Trinity I - Exhibit Hall

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