Evaluation Synergic Effects of Cigarette Smoke and Saliva

Objectives: The aim of this study was to evaluate the cytotoxic effects of CS on the human peripheral blood lymphocytes in the presence of stimulatory or non-stimulatory saliva in an in vitro model.

Methods: Ten healthy volunteers in age range of 25 to 29 were selected and samples of peripheral blood lymphocytes and saliva (whole and stimulated saliva) were collected from them. We used peripheral blood lymphocytes suspensions (PBS) and exposed them in 6 different medium. All samples were collected from all medium after 20 and 80 minutes. The survival rates of PBL were determined at 20 min and 80 min.

Results: After 20 minutes, PBS viability rate in 2 last groups had significant differences. After 80 minutes, PBS viability rate in 3 the last mediums showed significant difference. (p<0.05) The cell survival rates following exposure to CS in the presence of PBS supplemented with whole or stimulated saliva were significantly lower at 80 min in compare with 20 min (P<0.05).

conclusions:Tobacco effects are significantly increased in presence of saliva especially stimulated saliva.