Nanoleakage Expression in the Resin Cement-Dentin Interface

Objectives: The aim of this study was to evaluate the ability of two different resin cements in sealing human dentin and nanoleakage prevention. Methods: Resin composite overlays (Estenia) were fabricated and cemented to mid-dentin surface with either a universal self-adhesive resin cement (RelyX Unicem Maxicap, 3M ESPE) or a dual-cure resin cement with self-etching primer (Panavia F2.0, Kuraray Medical). After 24h storage in water, the specimens were sectioned, placed into 50% (w/v) ammoniacal silver nitrate solution for 24h, immersed into photo-developing solution for 8h and observed under FE-SEM and EDS. Ten interfacial images (n=10) from each material were obtained for which percentage distribution of metallic silver particles in the resin cement-dentin interface (h=2.6µ x w=23.5µ) were calculated with a digital image analysis software. The data were statistically analyzed by Kruskal-Wallis Test with statistical significance defined as p<0.05. Results: There was a significant statistical difference between the two resin cements. The mean±SD percentage of silver particle penetration for RelyX Unicem and Panavia F2.0 were 7.4±4.6 and 18.7±8.7, respectively. The Kruskal-Wallis Test mean ranks were 6.8 and 14.2, respectively. Panavia F2.0 showed clear silver uptake and higher peak on the elemental energy spectroscopy of EDS. High-magnification FE-SEM images after silver challenge are shown below. Conclusion: Different nanoleakage expressions were observed between the materials, in a short-term study.