Human salivary alpha-amylase in children with different caries indexes

Objectives: The aim of this study was to determine the differences of the quantified amounts of salivary alpha-amylase in the saliva of children with different caries indexes. Methods: Salivary samples were obtained via spontaneous salivation from a total of 100 children, which were divided into four groups (n=25) according to four index categories of ICDAS: sound, white opacity, microcavity and extensive cavity. The total quantity of protein reported in each of the samples was determined by use of the Bradford Method and the concentration of salivary alpha-amylase was determined by an indirect ELISA technique.Results: Non-parametric statistical methods needed to be applied. It was found that there is no significant statistical difference for the total protein variable in the four groups, while for the salivary alpha-amylase variable concentration there was a significant statistical difference between groups of individuals with white opacity and extensive cavities, between individuals with microcavity and extensive cavity, and between sound individuals and individuals with extensive cavity.

Conclusions: The results suggest that from the different functions identified for salivary alpha-amylase, protection of the tooth surfaces has major relevance.