Phototherapy promotes attachment and subsequent proliferation of human osteoblasts-like cells

Objectives:

Preliminary research has proved that light emitting diode(LED) revealed positive stimulatory effect on fibroblasts proliferation in vitro. The aim of the study was to investigate the effect of LED phototherapy on attachment and proliferation of human osteoblasts-like cells cultured on titanium implant material.

Methods:

Cells derived from human mandibular bone were exposed to LED at doses of 1 or 2 J/cm2 and then seeded onto titanium discs. Non exposed cultures served as controls. After 3 and 6 h, cells were stained and the attached cells were counted under a light microscope. In order to investigate the effect of LED on cell proliferation after 48, 72 and 96 h, cells were cultured on titanium specimens for 24 h and then exposed to LED for three consecutive days. Cell proliferation was determined after staining by counting cells under a light microscope. Furthermore, MTT analyses were also performed to determine cellular attachment and proliferation.

Results:

Cellular attachment was significantly enhanced (p <0.05) by LED irradiation at doses of 1 and 2 J/cm2 after 3 and 6 h. The proliferation assays showed higher cell proliferation (p <0.05) in irradiated group at doses of 1 and 2 J/cm2 after 72 and 96 h with agreement between cell counting and MTT analyses.

Conclusion:

It is concluded that in this cellular model the attachment and proliferation of human osteoblasts-like cells cultured on titanium implant material are enhanced by LED indicating that LED can modulate the activity of cells and tissues surrounding implant material.