Staining and Microleakage Evaluation of Re-sealing in Class V Restoration

Objectives:The purpose of this in vitro study was to evaluated the microleakage and superficial staining of composite restorations re-sealed with surfaces sealants after inhibition of oxygen layer. Methods:Forty cavities had been prepared for Class V restorations on buccal and lingual surfaces of twenty molars. The cavities were restored utilizing the adhesive system Clearfil SE Bond and the Renew composite. After the restorative procedure, finish and polish the teeth were randomly assigned in four groups. Group(B): re-sealed with a surface sealant Biscover. Group(O): re-sealed with a surface sealant OptiGuard. Group(BI): re-sealed with a surface sealant Biscover and inhibition of oxygen layer with a glicerin gel. Group(OI): re-sealed with a surface sealant OptiGuard and inhibition of oxygen layer with a glicerin gel. The groups were thermocycled (5000 cycles), impermeabilized, immersed in basic fucsine 0.5% (24h), washed and photographed to estimate the degree of superficial staining. The pictures were projected and the superficial stainings were classified as: absent (1), located (2) or generalized (3), and analized by the Kruskall Wallis test. The specimens were sectioned (4 slices). The slices were photographed, measured with the software Image Tool and analyzed by ANOVA. Results:For superficial staining the groups B and O had the highest staining (B: 30.5) (O: 30.5) than the groups BI (10.5) and OI (10.5). In comparison of the microleakage below the CEJ, there was no significant difference between the groups B (2.7 ± 0.23), O (2.9 ± 0.25), BI (2.6 ± 0.22), BO (2.8 ± 0.24) (P=1). Above the CEJ, there was no also significant difference between the groups B (1.49± 021), O (1.48 ± 0.20), BI (1.51 ± 0.23), OI (1.53 ± 0.25) (P=1). Conclusions: The inhibition of oxygen layer reduce the superficial staining of the composite restorations re-sealed with surface sealants. However, this procedure did not decreased the microleakage.