Study Osteogenic Induction of Pulp Stem Sells from Deciduous Teeth

Objectives: Obtained a distinctive population of multipotent stem cells from the remnant dental pulp from human deciduous incisors and proved their ability to differentiate to osteoblasts. Methods: we isolated and cultured dental pulp stem cells from human exfoliated deciduous teeth (SHED), and induced them to osteoblasts. First dental pulp were separated from a remnant crown and digested in a solution of collagenase type I and dispase ,then isolated and purified by limited dilution. We used several methods to assess colony-forming efficiency, draw cells growth curve , and characterize the immunophenotype of SHED by immunohistochemistry staining with antibodies of Vimentin,STRO-1 and CD44. Then induced the SHED to differentiate to osteoblasts, tested cells with calcified nodules staining, collagen type I staining and immunhistochemistry staining with osteocalcin. Results: Induced cells elicited a biological and morphologic characteristics similar to those of osteoblasts. Conclusion: Taken together, we obtained SHED and induce them to osteoblasts successfully.