Bone Regeneration with Small Intestinal Submucosa and nm TCP

Objective:Small intestinal submucosa(SIS) is a naturally occurring collagenous extracellular matrix which has been reported to obtain bioactive molecules. It has been used with success in many kind of tissues reparations. Some researches also showed that SIS can facilitate bone growth. This study was performed to investigate whether the small intestinal submucosa(SIS)/ nano meter crystal b - tricalcium phosphate(nm b-TCP) composite can enhance the regeneration of mandibular defect of the New Zealand rabbit.Methods: 26 mandibular defects of rabbits were made and randomly divided into 4 groups: group Ⅰ: single SIS was applied to each defects; groupⅡ: nm b-TCP was applied; groupⅢ: the composite scaffold material of SIS and nm b-TCP was applied; groupⅣ was used as control. Three months after the operation,the specimens were sacrificed for macrography, x-radiation,histological analysis, scanning electron microscopy observation,and also recorded the relative bone density(RBD). One-way ANOVA was used for statistical analysis. Results: The group used composite scaffold materials was found more newborn trabeculars with more mature formation,and the average relative bone density(RBD) is higher than the other 3 groups(p<0.05). The statistical results of mineral apposition rate (MiAR) and Bone-volunme fraction are the same as its of RBD.Conclusions: The composite materials of small intestinal submucosa and nano meter crystal b -tricalcium phosphate can enhance bone regeneration in mandibular defects of rabbits.