HGF and NHOK Showing Different Growth by Chitosan-PVP Hydrogel

Objectives: Many researchers are interested in wound healing in the treatment of burns, prevention of post surgical adhesions and cosmetic surgery by excess collagen production and scar formation. Synthetic epidermal substitutes with cultured epithelial cells seem to be an attractive strategy since keratinocytes have been demonstrated to modulate fibroblast growth and collagen synthesis. Bioabsorbable and biocompatible chitosan structurally mimics hyaluronic acid. Recently, a biocompatible synthesized chitosan-PVP(polyvinyl pyrrolidone) hydrogels demonstrated in vitro biocompatibility for biomedical applications. However, there is no report on this hydrogel's ability to modulate human gingival fibroblast growth. The purpose of this study were to investigate different growth modulation between human gingival fibroblast and normal human oral keratinocyte by chitosan-PVP hydrogel, and to apply this biocompatible synthetic polymer to oral and maxillofacial wound healing. We have synthesized a hydrogel from chitosan-PVP and examined its effect on human gingival fibroblast growth modulation in vitro.

Methods: Non-toxic and biocompatible hydrogel with human gingival fibroblasts and epithelial cells was tested by MTT assay.

Results: HGF showed a higher growth proliferation than that of NHOK after cell seeding. In MTT assay, 30% hydrogel leach out products showed a higher cellular viability in NHOK than that of any other products. In MTT assay, 30% hydrogel leach out products showed relatively lower cellular viability of HGF. In growth profile, NHOK showed about 7 folds higher than HGF after 1 day, while about 2 folds higher after 5 days. And also NHOK showed above about 70% cellular viability from 1 to 7 days.

Conclusions: It suggested that Chitosan-PVP hydrogel would inhibit relatively the growth of HGF and stimulate the growth of NHOK. This phenomenon may prove to be of use in wound management of oral and maxillofacial area as epithelial substitutes.

Key words : Different Growth, HGF, NHOK, Chitosan-PVP hydrogel