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Effect of HB-EGF on Wound-Healing in PDL
J.M. KIM1, Y.-J. YOO2, E.J. PAK1, and J.-H. CHA1, 1Yonsei Univerisity College of Dentistry, BK21 project, Oral Science Research Center, Seoul, South Korea, 2Yonsei Univerisity College of Dentistry,BK21 project, Oral Science Research Center, Seoul, South Korea | Objective: There is growing attention on periodontal regeneration and wound healing for periodontal therapy and replantation of avulsed tooth. This study is to investigate the effects of heparin-binding epidermal growth factor-like growth factor (HB-EGF) on cell repopulation and signal transduction in periodontal ligament (PDL) cell after scratch-wounding in vitro. Methods: Human PDL cells were obtained from the explant tissue of human healthy PDL. After wounding PDL cells, the change of mRNA expression of HB-EGF and epidermal growth factor receptor (EGFR) 1 to 4 was examined. The effects of HB-EGF on the PDL cell proliferation and repopulation were examined in vitro by MTT assay and photographing of injury, respectively. The phosphorylation of Erk1/2, p38 and Akt was determined by Western blot analysis. Results: Scratch-wound upregulated significantly mRNA expression of HB-EGF whereas the wound didn't change the expression levels of EGFR1 to 4. Interestingly, the expression of EGFR2 and 4 was not detectable before and after the wounding. Treatment of HB-EGF promoted PDL cell proliferation and repopulation. The scratch-wound stimulated phosphorylation of Erk1/2 and p38 but not Akt in PDL cells, and the treatment of HB-EGF after the wounding amplified the activations of Erk1/2 and p38 but not Akt. Furthermore, the inhibition of Erk1/2 blocked the process of cell repopulation induced by HB-EGF while the inhibition of p38 delayed the process. Conclusion: These results suggest that HB-EGF may be a critical factor in wound healing of human PDL cells by a mechanism that requires Erk1/2 activation via the specific interaction with EGFR1. This work was supported by the Korea Research Foundation Grant (KRF-2007-E00512). | Seq #95 - Periodontal Pathology Posters 2:00 PM-3:15 PM, Thursday, July 3, 2008 Metro Toronto Convention Centre Exhibit Hall D-E |
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