In Vitro Study of Dentinal Tubule Occlusion with Sol-gel DP-bioglass

Objectives:

DP-bioglass paste has been demonstrated to produce 60 µm of sealing depth on exposed dentinal tubules. However, the occlusive effect depended on a continuous placement of DP-bioglass paste on dentinal surface for three days. To fabricate highly reactive DP-bioglass particles, the sol-gel method was used together with different catalysts. And we compare the occlusive effect of sol-gel bioglass with two commercial products.

Methods:

Smaller bioglass particles via sol-gel processing were fabricated and utilized HNO3, NaOH, and H3PO4 as catalysts. After that, dentin disc specimen were prepared and treated with prepared sol-gel DP-bioglass, One Coat Bond (Coltne/Whaledent Inc., NJ, USA) and Seal & Protect (Dentsply Caulk, Milford, DE, USA). All specimens were examined under SEM.

Results:

The application time of DP-bioglass paste was significantly reduced to 10 minutes. Percentage of tubular occlusion with DP-bioglass catalyzed with HNO3, NaOH, and H3PO4 were 65.4%,53.2% and 54.0% respectively, while One Coat Bond and Seal & Protect yielded 51.3% and 41.2% respectively. Further, the average depth of tubular occlusion with DP-bioglass catalyzed with HNO3, NaOH, and H3PO4 were 62.7 microm, 58.9 microm and 55.8 microm respectively was 55.8-62.7 microm, while One Coat Bond and Seal & Protect produced 40.8 microm and 32.5 microm respectively.

Conclusions:

The best sealing performance of tubular occlusion was rendered by DP-bioglass catalyzed with HNO3. Therefore, it was considered to exhibit the greatest potential in treating dentin hypersensitivity.