Bisdemethylcurcuminoid effects on IL-6 and PGE2 Production from Gingival Fibroblasts

Objectives: Curcuminoids are a group of naturally occurring phytochemicals which exhibit a variety of pharmacological effects such as anti-inflammatory activity. Therefore, the effect of bisdemethylcurcuminoid on inflammatory cytokine production by human gingival fibroblasts was studied.

Methods: To determine the amount of interleukin-6 (IL-6) and prostaglandin E2 (PGE2) in the cell culture supernatants from interleukin-1 β (IL-1β)-stimulated human gingival fibroblasts in the presence or absence of bisdemethylcurcuminoid. Fibroblast cultures were established from healthy gingival tissue donors and were challenged with IL-1β in the absence and presence of bismethylcurcuminoid. COX-2 inhibitors and IL-6 were also tested to support the findings. After 24 hrs of incubation, culture supernatant were collected and analyzed for IL-6 and PGE2 content by ELISA.

Results: Bisdemethylcurcuminoid inhibited the production of IL-6 by IL-1β-stimulated gingival fibroblasts in a concentration dependent manner. It inhibited IL-6 and PGE2 production by % control of 39.92-59.69 and 14.52-43.28, respectively. Cell viability was not significantly affected in this treatment.

Conclusions: These findings clearly showed the regulation of IL-1β-induced IL-6 and PGE2 production by bisdemethylcurcuminoid, and suggest their use in the treatment of periodontal diseases and other inflammatory diseases.