The Inhibitory Effect of Platinum Nanoparticles on Oxidative Cytotoxicity

OBJECTIVES: Reactive oxygen species (ROS) is associated with many inflammatory diseases. ROS cause tissue damage by a variety of mechanisms; DNA or protein damage, lipid peroxidation, oxidation of important enzymes, stimulation of pro-inflammatory cytokines. IL-8, one of important chemokines, is induced by ROS in several cells. In this study, we investigated the effect of Platinum nanoparticels, a novel antioxidant agent, on H₂O₂ stimulated IL-8 production in oral epithelial cell line KB.

METHODS: Cell cultures with 0, 10, 100µM Platinum nanoparticles (manufactured by APt Co., LTD., Tokyo, JAPAN) were challenged with 500µM H₂O₂ respectively. After 24 hours incubation at 37°C in 5% CO₂, cells were subjected to cytotoxity test (WST assay) and IL-8 assay (ELISA). Statistical significance of differences between groups was analyzed by one-way analyses of variance (ANOVA) and followed by Tukey's test.

RESULTS: H₂O₂ induced the dose-dependent cytotoxicity to KB cells. After 500µM H₂O₂ treatment, cell viability was 58.7% compared with control. Although the basal level IL-8 was 320pg/mL, it reached 2610pg/mL after H₂O₂ treatment. These ROS effects were significantly reduced by Platinum nanoparticles (p<0.01). Especially, the H₂O₂ induced cytotoxicity and IL-8 production was completely neutralized by 100µM Platinum nanoparticles.

CONCLUSIONS: Platinum nanoparticles inhibited IL-8 production in oral epithelial cells stimulated by H₂O₂. Platinum nanoparticles are expected as a novel anti-inflammatory agent.