GCF Levels of IL1-b,IL-6 and TNF-a in Papillon Lefevre Syndrome

Objectives: Papillon Lefevre Syndrome is a rare autosomal recessive disorder in which premature loss of teeth due to a severe periodontitis and palmoplantar hyperkeratosis occurs. The cytokines Interleukin-1beta (IL-1β), Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-6 (IL-6) are inflammation-related cytokines associated with bone resorption in periodontal disease. The aim of this study was to investigate the levels of IL-1β, TNF-α and IL-6 in gingival crevicular fluid (GCF) samples collected from patients with PLS.

Methods: Nine patients with PLS, and 20 healthy control (C) subjects were selected for the study. The gingival crevicular fluid was collected by using sterile filter papers. Assays for GCF IL-1β, TNF-α, and IL-6 were carried out by an enzyme-linked immunosorbent assay (ELISA) method. The groups compared with each other by utilizing Mann Whitney U-test.

Results: The GCF levels of IL-1β and IL-6 were lower in PLS group compared to those of the control group (p<0.05). TNF-α levels of PLS group did not differ from the controls.

Conclusions: These results suggest that the cytokine profiles of PLS differ from the controls. The lower expression of IL-1β and IL-6 might explain the deficiency in the immune response of PLS patients. The lack of a sufficient host response may be the cause of the tissue destruction.