Direct-pulp-capping Effect of Experimentally Developed adhesive-resin-systems Containing Reparative Dentin-promoting-agents

Objective: In this study examined the effects of various experimentally developed adhesive resin systems containing four reparative dentin promoting agents on the wound healing process of exposed rat pulp.

Methods: The twelve experimental groups (MB1~MB12) were capped with a combination of the following primers and bonding agents: commercially available Mega Bond^® primer (Kuraray, MBP) and Mega Bond^® bonding agent (MBB), and experimental MBB containing each quantity of calcium phosphate (Hydroxyapatite, Brushite, Whitlockite, Octacalcium Phosphate). The control group was capped with Dycal^®(Dentsply Sankin) and MBB (CONT). The sample number of each group was 5. The rats were sacrificed on the 3, 7, 14th post-operative day. The specimens were alternately stained with H-E, Hucker-Conn bacterial stain, Reticulin silver impregnation and the sABC method on TGFb1 and DMP1.

The followings: pulp tissue disorganization (PTD), inflammatory cell inflammation (ICI), reparative dentin formation (RDF), and bacterical penetration (BP) were microscopically evaluated. The findings were graded using four legend scales. Each result was statically analyzed using the Kruskal-Wallis H-test and then Mann-Whitney U-test as a post-hock test (P<0.05). The correlation of ICI and BP was also examined by the Kendall correlation analysis (P<0.05).

Results: Reparative dentin was detected in all expepimental groups including CONT. Of the twelve experimental groups, MB5 (Whitlockite 5wt%) made most quantity of reparative dentin.

Conclusions: Whitlockite is useful material for reparative dentin promoting agents.