Effect of Chloromycetin and Normal Saline on Periodontal Ligament Cells

Objective: The aim of this study was to investigate the proliferation and ultrastucture of cultured periodontal ligament cells after exposure to 0.25% chloromycetin and normal saline. Methods:Human periodontal ligament cells were exposed for 1h to 0.25% chloromycetin , normal saline and DMEM culture medium. The number of viable cells were counted immediately after exposure(0h) and at 12,24and 48h,to test the proliferative capacity of the cells after treatment.The ultrastucture was observed throught transmission electron microscope. The results were analyzed with ANOVA using SAS9.1 software package. Results: At 0,12,48h,after exposure to the solutions, the DMEM culture medium group has the highest number of viable cells among the test groups(P<0.0001). There was no difference in the number of viable cells between 0.25% chloromycetin and normal saline at any time point(P>0.05). Most of mitochondrions changed to vacuole after the cells exposed to 0.25% chloromycetin,and the number of endoplasmic reticulum decreased. Conclusions: The effect of chloromycetin on proliferation in human periodontal ligament cells is same as the effect of normal saline,but chloromycetin causes damage to the ultrastructure of human periodontal ligament cells.