ABSTRACT: 3386

Fluoride and oxidative status: acute experiment in rats parotid glands

P.M. YAMAGUTI, E. GANZERLA, A. SIMOES, F.N. NOGUEIRA, and J. NICOLAU, University of Sao Paulo, School of Dentistry, Sao Paulo, SP, Brazil

Many reports have proposed that fluoride in varying concentrations induces increased reactive oxygen species generation, enhanced lipid peroxidation (LP) and impaired antioxidant enzyme defence system (AEDS) in blood and tissues of experimental animals causing several biochemical alterations. Although the most pronounced effects of fluoride intake are manifested in bones and teeth, soft tissues are also affected.

Objectives: Due to a paucity of reports investigating short- and long-term effects of varied low- and high-dose fluoride intake on soft tissues, specifically salivary glands, this study aimed to evaluate effects of low-dose sodium fluoride (NaF) acute exposure on AEDS and LP in parotid salivary glands (PG) of rats.

Methods: 2-month-old male Wistar rats were injected intraperitoneally NaF solution (F_#) (15mgF^-/kg b.w.). Control (C_#) group was administered same volume sodium chloride solution (3.05%). Animals (n=6) were euthanized 1,3,6,12 and 24 hours after injection. PG were extracted and analyzed for superoxide dismutase (SOD) and catalase activities, malondialdehyde and proteins levels, and statistically analyzed (ANOVA, Tukey’s, p<0.05).

Results: Low-dose and short-term NaF treatment did not cause significant differences in activities of SOD and catalase, levels of malondialdehyde and proteins in the PG homogenates within any of the studied groups. Although a trend to enhanced LP was observed in time-dependent manner.

Groups

SOD (U/mg-prot)

Catalase (mU/min/mg-prot)

Malondialdehyde (µg/mg-prot)

Proteins (mg-prot/ml)

p<0.05

C₁(6)

7.40±0.88

5.99±0.37

2.29±0.69

11.63±1.11

n.s.

F₁(6)

6.50±1.19

5.39±0.29

2.22±0.71

12.50±0.99

n.s.

C₃(6)

7.12±1.76

5.47±0.61

2.30±0.55

14.24±1.70

n.s.

F₃(6)

6.41±1.61

4.77±0.74

3.20±0.70

14.34±2.42

n.s.

C₆(6)

6.90±1.16

5.24±0.75

2.28±0.65

11.70±2.01

n.s.

F₆(6)

4.72±1.15

5.17±0.82

3.07±0.45

12.88±2.57

n.s.

C₁₂(6)

5.99±1.16

5.46±1.10

2.43±0.36

14.50±1.92

n.s.

F₁₂(6)

6.64±0.98

4.96±0.90

3.00±0.49

12.44±1.10

n.s.

C₂₄(6)

7.11±1.85

5.89±1.33

2.38±0.41

13.33±0.47

n.s.

F₂₄(6)

6.37±1.55

5.34±1.10

2.86±0.40

12.75±1.36

n.s.

Mean±S.D.(n=6); p<0.05; n.s.=non-significant

Conclusions: Results suggest that acute exposure to low-dose administration of NaF did not interfere with AEDS or LP in PG of rats.

Seq #308 - Saliva
1:45 PM-3:00 PM, Saturday, July 5, 2008
Metro Toronto Convention Centre Exhibit Hall D-E

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