Cytotoxic effects of ionomers glass in cell cultured

Objectives: We undertook this study to evaluate the cytotoxicity of Ketac Molar easymix-3M; Maxxion R- FGM; Riva Self Cure-SDI ; Vitro Molar-DFL using in vitro assays. Methods: We used the NIH 3T3 cell line (ATCC CRL 1658) for available cell viability and pulp fibroblasts (FP1). The gel was directly applied to coverslips that were placed in contact with the cultured cells. The control group was cultures that received plain coverslips. In long-term assay (cell survival), the experimental periods were 1, 3, 5, and 7 days, and in short-term assay (cell viability), the periods were 0, 4, 8, and 12 hrs. We determined cell number and cell viability (%) by counting the cells in a hemocytometer, using the Trypan blue dye exclusion assay and MTT. Each datapoint corresponds to mean ± SEM of either cell number or cell viability (%) from three dishes. The data were compared by the Kruskal-Wallis test (GMC Basic Software, version 7.1). The level of significance was 5% (p ≥ 0.05). Results: The data analyses obtained in our experiments allowed us to observe that groups treated with the materials after polymerization had viable cells numbers and percentages lower than those of the control group, but allowed for more long-term cellular growth. Conclusion: Our study established the reduced cytotoxicity the materials after polymerization, in fibroblast culture tests.