Candidosis Associated with Maxillary Complete Denture: Demographic, Clinical, Microscopic Evaluation

Denture stomatitis is an inflammatory process affecting the oral mucosa in contact with acrylic resin of complete dentures or removable partial dentures.Objectives: demographic, clinical and microscopic analysis of candidosis associated with the use of maxillary complete denture, of the atrophic and papillary hyperplasic forms and associated with inflammatory fibroepithelial hyperplasia. Methods: Archives microscopical samples from Bauru Dental School, were reviewed in sections stained with hematoxylin and eosin, Grocott's methenamine silver and periodic acid-Schiff, and compared to normal palatal tissues obtained from non-denture wearers.Results: Demographic analysis were observed from aged between 51 to 60 years,females and white individuals. The clinical analysis was characterized by:red points in palatal for atrophic form, a volume increase in the central area of palate for papillary hyperplasic and a volume increase on the peripheral region of the complete denture associated with inflammatory fibroepithelial hyperplasia.Microscopic analysis presented different from the normal mucosa, most cases of oral candidosis present hyperplasic epithelial lining with elongated rete pegs, some with “test tube” aspect, with frequent areas of absence of keratin, small foci of lichenoid reaction and microabscesses, as well as extensive areas of pseudo epitheliomatous hyperplasia. Only papillary hyperplasic lesions exhibited bifid rete pegs, besides high frequency of keratin pearls-like and long “test tube” rete pegs, when compared with the other forms of oral candidosis. The connective tissue underlying the oral candidosis presented moderate to intense mononuclear inflammatory infiltrate, predominantly at the lamina propria and perivascular region. Fungal hyphae and yeasts were not identified by special staining with Grocott's methenamine silver and PAS. Thus, the microscopic diagnosis of oral candidosis should not depend on the visualization of fungal hyphae and yeasts at the most superficial layers of epithelial lining.Conclusions: For all analysis described could be able observed differences between an oral mucosa with candidosis and a normal oral mucosa.