Media Effect on Bond Strenght of a Composite to Dentin

OBJECTIVES: The purpose of this study was to evaluate the influence of storage media on the micro tensile bond strength with a total etching adhesive system to dentin. METHODS: A flat occlusal dentin surface was exposed using wet 180, 240, and 320-grit SiC paper on caries-free human molar teeth. Adhesive (Optibond Solo Plus) was applied followed by 6 mm of composite (Premise) applied in 2 mm increments. Four beams (0.8 mm2) were obtained from the most central area of twenty-five teeth for each group, labeled to indicate the position of the beams and stored in either water or artificial serum at 37 degrees C for 7 months. Once a month, the storage medium was exchanged in an aseptic manner. Microtensile dentin bond strengths were determined using a non-trimming technique. Statistical comparisons between adhesive-storage combinations were performed using one way ANOVA. RESULTS: The microtensile bond strength of the specimens stored in water (MPa 50.8 ± 15.3) was statistically significant greater than those stored in artificial serum (MPa 32.3 ± 11.7). CONCLUSION: Artificial serum decreased microtensile bond strength to a greater extent than water