Evaluation of the Anticariogenic potencial of an experimental GIC

Objective: The aim of this work was to evaluate the in vitro fluoride release, uptake ability, and the inhibitory activity dealing with the glass ionomer cements (GIC): Vitrebond (3M ESPE), Ionomaster F (WILCOS), and also an experimental GIC. Methods: The fluoride release ten specimens were fabricated according to the instructions of the manufacturer for each of the five experimental groups, GI (Vitrebond), GII (Ionomaster), GIII (Powder Experimental + Liquid Vitrebond), GIV (Powder Experimental + Liquid Ionomaster), GV ((Powder Experimental + Liquid Experimental). All of the specimens were subjected to a pH cycling model, during 15 days. Afterwards, all the specimens were subjected to the application of acidulated phosphate fluoride (APF, 1.23% at pH 3.6-3.9) for four minutes. Thereafer the specimens were washed and subjected to the same pH cycling for additionally 15 days. The solution fluoride concentration was determined during a period of 30 days. For the microbiological test, the five experimental groups were evaluated on S. mutans, L. acidophilus, and A.viscosus using the agar diffusion testing. The inocula were obtained by seeding the bacterial strains into BHI medium and then were incubated at 37o C for 24 hours. A 0.2% chlrorexidine digluconate solution (GVI) was used as the control. The experimental method was repeated in ten Petri dishes, incubated at 37o C, for 24, 48, and 72 hours. The inhibition zones around the wells were subsequently measured. Results: There was statistically significant difference among materials. Conclusions: This study revealed a higher fluoride release for Ionomaster F, followed by the Vitrebond, along with similar features for the three groups using the experimental cement; all the experimental groups presented a rise in fluoride release upon uptake, despite the different release features.The antibacterial activity for two CIVMRs was highly significant. Vitrebond presented the best antibacterial activity on A. Viscosus and L. Acidofilus.