ABSTRACT: 0451

Biofilm architecture on feldspar ceramic by confocal laser scanning microscopy

A.S.B. BRENTEL A.S¹, K.Z. KANTORSKI², S.M.B. PEREIRA², S.B.P.D. FUCCIO³, L.F. VALANDRO⁴, R.O.A. SOUZA⁵, and M.A. BOTTINO⁶, ¹University of são paulo state, São Jose Dos Campos, Brazil, ²Paulista State University, São José dos Campos, Brazil, ³Unicamp University, Piracicaba, Brazil, ⁴Paulista University, São José dos Campos, Brazil, ⁵Sao Paulo State University, Sao Jose dos Campos, Brazil, ⁶São Paulo State University (UNESP), São José dos Campos, Brazil
Objectives: This study evaluated the biofilm formed in situ on ceramic using confocal laser microscopy. Methods: Standardized specimens of a feldspar ceramic (VM7, Vita) were made according to manufacturer specifications and submitted to the following experimental conditions according to finishing and polishing protocols (n=10): a) glaze; b) glaze and diamond bur; c) glaze, diamond bur and rubber point; d) glaze, bur diamond, rubber point and felt disks impregnated diamond paste. Five healthy volunteers used intra-oral devices with the ceramics specimens for 8 hours. After experimental trial, specimens were removed and undisturbed biofilm was analyzed by the non- invasive technique of confocal microscopy (CLSM). Analysis of variance One Way and tukey test (p > 0.05) was used for to compare the date. Results: None statistical difference was observed among the groups G1 and G4, G2 and G3 respectively, regarding biomass (Q= 11,764 and 0,9163 ) , regarding thickness (Q= 0,9539 and 0,3696 ) and regarding roughness coefficient (Q= 0,8596 and 25,221 ) of biofilm. Conclusions:Thus conclude that there showed a similar relationship with the data among the groups G1 e G2, G1 e G3, G2 e G4, G3 e G4.
Seq #75 - Antibacterial/Caries-inhibiting Effects, Biomaterials, and Bioengineering 2:00 PM-3:15 PM, Thursday, July 3, 2008 Metro Toronto Convention Centre Exhibit Hall D-E
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