Cellular effect of nano and macrocoatings on cpTi surfaces

Objectives: This study presents the characterization of cpTi metal surfaces modified by procedures such as polishing (P), polishing and coating with TiO2 (PC), sandblasting and coating with TiO2 (SC), polishing and coating with TiN (PN) and coating with TPS (TPS) and evaluates their effect on proliferation of fetal human osteoblast cells (hFOB 1.19).

Methods: Al2O3 particles were used in sand blasting and coating of the surfaces was done by physical vapor deposition (PVD) technique. TPS surfaces were supplied by Friadent (Friadent, Mannheim, Germany). These five surfaces were characterized quantitatively by a non-contacting optical profilometer in terms of Rz and Ra roughness values and qualitatively by SEM micrographs. hFOB 1.19 were incubated at these surfaces for 24 hours and they were evaluated in terms of mean total cell counts. Cells' morphologies were evaluated qualitatively by SEM micrographs. P surfaces served as control group (CG)for the profilometry and SEM evaluations. Glass discs served as CG for the cell culture. Values obtained were analyzed statistically by Kruskall Wallis test and Post Hoc LSD test.

Results: P, PN, and PC surfaces statistically had no difference in terms of Ra values (pP-PN=0.074, pP-PC= 0.029, and pPN-PC= 0.663). PN and PC surfaces statistically had no difference in terms of Rz values (p=0.864). Highest mean total cell counts were found for the CG (42.41±7.10x104 cells/ml) followed by the TPS group (37.50±5.06x104 cells/ml). Lowest mean total cell counts were found as 25.66±5.22x104 cells/ml for the SC surfaces. The results revealed no correlation between surface roughness and the mean total cell counts.

Conclusions: These coated surfaces in our study appear to favor cellular attachment of human fetal osteoblast cells.