Fluocinolone Acetonide effects on Pulp Cell Proliferation and Collagen Synthesis

Objective: The aims of this study were to investigate the effects of Fluocinolone Acetonide (FA) on human pulp cell proliferation and collagen type I synthesis.

Methods: Human pulp cells were seed in complete DMEM plus 10% FCS for 24 hours. Cells were then treated with various concentration of FA (50.0, 10.0, 1.0, 0.1 然). The MTT assay was performed to examine the proliferation of pulp cells at 24, 48 and 72 hours. The type I collagen synthesis by human dental pulp cells, treated with various concentration of FA (10.0, 1.0, 0.1 然), was examined by the Western blot analysis at 5 days. Reverse transcription polymerase chain reaction (RT-PCR)was performed to confirm the synthesis.

Results: FA showed significantly increased cell proliferation (p<0.05) at 10.0, 1.0 and 0.1 然 only in 48 and 72 hours groups. The result from Western blot analysis showed that 1.0 and 10.0 然 of FA significantly stimulated the synthesis of collagen for about 2-fold (p<0.05). This result was confirmed by reverse transcription polymerase chain reaction (RT-PCR) which indicated that 1 然 FA could significantly induce the expression of type I collagen mRNAs for about 2.8 times (p<0.05).

Conclusion: The results demonstrated that FA enhanced pulp cell proliferation and type I collagen synthesis. These results suggested that FA may be the potential substance as a pulp capping material. This research was supported by Ratchadaphisek Somphot Endowment for Research Fund.