Antibacterial activity against MRSA from

Objectives: MRSA has been emerging worldwide as one of the most important problems in dental and medical fields. Therefore, new agents are needed to treat MRSA from acute oral infection. Methods: The antibacterial compound was successfully isolated from the chloroform fraction of methanol extract of sophora flavescens (S. flavescens) by repeated silica gel chromatography, and its structure was elucidated as chalcones (kuraridin and kuraridinol) by instrumental analysis using 1D-NMR, 2D-NMR and ESI/LC/MS. Results: The CHCl ₃ fraction from solvent partition possessed greater antibacterial potential than other fractions. Their structures were identified kuraridin and kuraridinol by MS, ¹H-NMR, and ¹³C-NMR. The minimum inhibitory concentrations (MICs) of chalcones to the S. aureus (MRSA and MSSA) ranged from 2 to 64 µg/mL. Among chalcones, kuraridin and kuraridinol showed significant antibacterial activity against MRSA (8 µg/mL). Conclusion: These results suggest that chalcones might have the antibacterial activity against MRSA from acute oral infection.